ELECTROPHORETIC ANALYSIS OF YEAST EXTRACTS

Electrophoretic Analysis of Yeast Extracts

Electrophoretic studies of sonic extracts of Proteus vulgaris. I. Effect of growth environment on electrophoretic patterns.

The analytical electrophoretic pattern of the buffer-soluble proteins extracted from bacterial cells is both characteristic and descriptive of the organism (Hess et al., 1957; Wagman et al., 1958; Rodenberg, 1958). Further, a relatively large fraction of the material of the cell can be studied by the electrophoretic method. Hess et al. (1957) using successive extractive procedures on three stra...

Electrophoretic studies of red cell extracts of stored blood.

It has been shown that red cells of blood collected in acid-citrate-dextrose (ACD) undergo changes in their dimensions, osmotic fragility, permeability and metabolism during storage at 4° C (1). Information concerning the effect of storage on the physical and chemical states of soluble red cell proteins, particularly hemoglobin, is not available. Such data may be of interest in view of the mark...

Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera.

Serodiagnosis of cytomegalovirus (CMV) infection by complement fixation tests depends on showing a fourfold rise in antibody titer from acute- to convalescent-phase sera. Freeze-thaw and glycine-extracted, infected cell culture antigens used for these tests give markedly different titers in reactions with the same sera. In this study, we characterized the CMV-infected cell polypeptides containe...

The identification of pathogenic yeast strains by electrophoretic analysis of their chromosomes.

Epidemiological studies require characterisation of pathogenic yeasts at and below the species level. The chromosomes of 130 strains of four pathogenic species of the genus Candida, isolated from clinical material, were separated by pulsed field electrophoresis with the clamped homogeneous electric field (CHEF) technique. Each species was characterised by a distinct electrophoretic karyotype (E...